Description
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to LF/LTF. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated LF/LTF. The competitive inhibition reaction is launched between with HRP-conjugated LF/LTF and LF/LTF in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LF/LTF in the sample. The color development is stopped and the intensity of the color is measured